|
KMID : 0624620080410020170
|
|
BMB Reports
2008 Volume.41 No. 2 p.170 ~ p.175
|
|
|
Protein transduction of an antioxidant enzyme: subcellular localization of superoxide dismutase fusion protein in cells
|
|
Kim Dae-Won
Kim So-Young
Lee Sun-Hwa
Lee Yeum-Pyo
Lee Min-Jung
Jeong Min-Seop
Jang Sang-Ho
Park Jin-Seu
Lee Kil-Soo
Kang Tae-Cheon
Won Moo-Ho
Cho Sung-Woo
Kwon Oh-Shin
Eum Won-Sik
Choi Soo-Young
|
|
|
Abstract
|
|
|
|
In protein therapy, it is important for exogenous protein to be delivered into the target subcellular localization. To transduce a therapeutic protein into its specific subcellular localization, we synthesized nuclear localization signal (NLS) and membrane translocation sequence signal (MTS) peptides and produced a genetic in-frame SOD fusion protein. The purified SOD fusion proteins were efficiently transduced into mammalian cells with enzymatic activities. Immunofluorescence and Western blot analysis revealed that the SOD fusion proteins successfully transduced into the nucleus and the cytosol in the cells. The viability of cells treated with paraquat was markedly increased by the transduced fusion proteins. Thus, our results suggest that these peptides should be useful for targeting the specific localization of therapeutic proteins in various human diseases. [BMB reports 2008; 41(2): 170-175]
|
|
|
KEYWORD
|
|
|
MTS, NLS, Protein therapy, Protein transduction, Subcellular localization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|